Chemistry of polyhalogenated nitrobutadienes, 17: Efficient synthesis of persubstituted chloroquinolinyl-1H-pyrazoles and evaluation of their antimalarial, anti-SARS-CoV-2, antibacterial, and cytotoxic activities

• Viktor A. Zapol’skii,
• Isabell Berneburg,
• Ursula Bilitewski,
• Melissa Dillenberger,
• Katja Becker,
• Stefan Jungwirth,
• Aditya Shekhar,
• Bastian Krueger and
• Dieter E. Kaufmann

Beilstein J. Org. Chem. 2022, 18, 524–532, doi:10.3762/bjoc.18.54

• of the malaria parasite P. falciparum (3D7 strain) using the SYBR Green I-based fluorescence assay [35]. Table 1 summarizes the activity of the compounds against the red blood cell stages of P. falciparum with EC50 values ranging from high nanomolar (200 nM) to low micromolar concentrations (4 µM

Halogen-containing thiazole orange analogues – new fluorogenic DNA stains

• Aleksey A. Vasilev,
• Meglena I. Kandinska,
• Stanimir S. Stoyanov,
• Stanislava B. Yordanova,
• David Sucunza,
• Juan J. Vaquero,
• Obis D. Castaño,
• Stanislav Baluschev and
• Silvia E. Angelova

Beilstein J. Org. Chem. 2017, 13, 2902–2914, doi:10.3762/bjoc.13.283

• .). Simulated TDPBE0 spectra in methanol. Electron density (isovalue = 0.002) mapped with electrostatic potential (color scheme: green for negative surface map values and blue for the positive ones). Chemical structures of TO and SYBR Green I – commercial monomethine fluorescent dsDNA binders. Synthesis of the

Terminal lipophilization of a unique DNA dodecamer by various nucleolipid headgroups: Their incorporation into artificial lipid bilayers and hydrodynamic properties

• Emma Werz and
• Helmut Rosemeyer

Beilstein J. Org. Chem. 2015, 11, 913–929, doi:10.3762/bjoc.11.103

• formation at a lipid bilayer–water phase boundary using SYBR Green I staining. For this purpose, various nucleic acids of different length and sequence were lipophilized by appending a terminal non-nucleosidic lipid head group and then bound on the surface of an artificial lipid bilayer. Subsequently

• , equimolar amounts of complementary and non-complementary DNA strands as well as a SYBR Green I solution were added to the bilayer-bound lipo-oligonucleotide. The kinetics of the ternary complex formation at the bilayer surface as well as the stability of the complexes against perfusion was measured by

A small azide-modified thiazole-based reporter molecule for fluorescence and mass spectrometric detection

• Stefanie Wolfram,
• Hendryk Würfel,
• Stefanie H. Habenicht,
• Christine Lembke,
• Phillipp Richter,
• Eckhard Birckner,
• Rainer Beckert and
• Georg Pohnert

Beilstein J. Org. Chem. 2014, 10, 2470–2479, doi:10.3762/bjoc.10.258

• also used for probing in life science comprises the heterocyclic thiazoles. This structural element can be found in commercial products, such as thiazole orange, SYBR® Green I or TOTO®, which are, e.g., used for DNA labeling. In these compounds the thiazole ring is part of a benzothiazole. We set out

Specific DNA duplex formation at an artificial lipid bilayer: fluorescence microscopy after Sybr Green I staining

• Emma Werz and
• Helmut Rosemeyer

Beilstein J. Org. Chem. 2014, 10, 2307–2321, doi:10.3762/bjoc.10.240

• the formation of stable DNA duplexes at the bilayer surface. This could be verified by Sybr Green I double strand staining, followed by incubation periods and thorough perfusions, and was visualized by single molecule fluorescence spectroscopy and microscopy. The different bilayer-immobilized

• complexes consisting of various DNA duplexes and the fluorescent dye were studied with respect to the kinetics of their formation as well as to their stability against perfusion. Keywords: artificial lipid bilayers; lipo-oligonucleotide duplexes; nucleic acids; Sybr Green I; Introduction The post

• (2b) – prepared by using compound 2a – was proven by fluorescence microscopy. Now, we simplify this technique by hybridizing an unlabelled DNA target strand to the bilayer-immobilized lipo-oligonucleotide and by using Sybr Green I (3, SG) [20][21][22] as a fluorescent double strand indicator [20][21